Application Of Molecular Genetics To Crops

Application Of Molecular Genetics To Crops The science of biotechnology is likely to be to the first half of the 21st century what the computer was to the second half of the 20th century. Its implications are profound, its potential benefits massive. Britain is well placed to keep our lead in Europe. I want to make it clear: we dont intend to let our leadership fall behind and are prepared to back that commitment with investment. are the words of Tony Blair at the European Bioscience Conference (November 2000) (cited in Francesca Tencalla, 2005), which is the best way to present my positive answer towards the question. The molecular genetics positively impacts the plant breeding paradigm. Introduction: Centuries ago, the science was unknown to the farmers but the present agricultural scenario is changing its traditional frame of conventional breeding to the most recent and modernized frame of molecular plant breeding. Today, the world agriculture is facing great challenges on four fronts visually, Production, Population, Pollution, and Politics. Also, the change in climate (global warming) is the next major challenge for the crops, as unlike animals they can not move from one place to another. The prospects for feeding humanity as we are in 21st century often are portrayed in a daunting light to keep pace with the population growth. As we are told that worlds population has been growing faster than crop production, since the early 1980s and is expected to be 8 billion in next or two decades. At this point, we need to think in-depth about the opportunities to meet these challenges and to improve the crop production at a subsequent rate. Are the agronomic practices merely going to pl ay the key role or the refined crop protection measures will be the major part of increase in crop production, at this junction of scientific development? No other than, molecular genetics and the application of molecular techniques to enhance the efficiency of plant breeding, will play the crucial role in crop improvement, is a promise to the next generations (Ben Miflin, 2000). At the turn of the 20th century, the discovery of principles of natural selection and hybridization by Darwin and the rediscovery of Mendelian genetics served as the basis for plant breeding and genetics. In the same way, tremendous increase in crop yields in the 21st century has been powered by the development of plant biotechnology, -omics era of molecular biology, and molecular genetics. The recent techniques of molecular genetics are emerging continuously to overcome the demand of growing population (Moose et al., 2008). The chief objective of molecular genetics is to enhance crop production and crop produce usefulness by genetic modification of crop plants; this is precisely what plant breeding has been doing from the day prehistoric man began to domesticate the orphan (wild) species. The molecular genetic techniques aim to give traditional breeding a technological boost, says Jorge Dubcovsky, a wheat molecular geneticist from University of California, whose group is presently working on Marker Assisted selection in wheat for 23 separate traits, conferring resistance to insect-pests, fungi and viruses. His enthusiastic claim is that this research could offer the wings for crop improvement to plant breeding what jet engine has brought to air travel. Basic Terms: Plant Breeding is an art as well as science of improving genetic makeup of plants in relation to their economic use (Paul et al., 2006; Singh B.D., 2003). Molecular genetics refers to the study of molecules, our genes, their structure and functions at molecular level. Also studies the transfer of genes from one generation to the next. It employs the principles of genetics and methods of molecular biology (NCBI, Wikipedia). Recently, Crop improvement is the science of value addition to the existing crop species by using the technique, which marries conventional plant breeding with molecular biology, to get theatrical gains in yield (Austin R.B., 1986). Hundred years of Genetics: (From domestication to transformation) Plant breeding deals with the aspect of crop production. In early days, plant breeding was mainly based on skills and the abilities of the breeder involved in the programme. But as the genetic engineering and the tools of molecular genetics were elucidated, breeding methods and programmes were designed in their light. Plant breeding began with the domestication, when primitive men cultivated the first crop for the benefit of mankind. Thereafter, the process of exploiting the crops for improvement of their commercial values has a long history in itself. As early as 700 B.C., Babylonians and Assyrians performed artificial pollination in date palm. Then, the first artificial hybrid named Fairchilds mule produced by Thomas Fairchild by crossing carnation with sweet William in 1717. These provide the clear evidence of plant breeding exist as an art before the discovery of Mendelian genetics. In 1900, the rediscovery of Gregor John Mendels paper provided the foundation for the vast knowledge of genetics for crop improvement. A noteworthy development resulted from the discoveries of G.H. Shull on inbreeding in maize (Zea mays), led to the production of hybrid varieties in maize, sorghum, cotton, rice and several other crops. Green Revolution, one of the greatest achievements in the modern plant breeding has been the introduction of dwarf gene in cereals, particularly in wheat (Triticum aestivum) and rice (Oryza sativa), (Borlaug, 2000). In 1960, Allozymes the first biochemical genetic markers were on hand. The next decade provided new tools to geneticists of restriction fragment length polymorphism (RFLP) and Southern blotting. Taq polymerase was found in 1980 and shortly Polymerase chain reaction was developed, which is now routinely used in plant breeding research. The recently developed technology is single nucleotide polymorphic markers based on high density DNA arrays, a technique known as Gene Chips (Rodomiro Ortiz, 1998). In 1980, the era of biotechnology began with the successful production of the first transgenic plant using Agrobacterium (Moose and Mumm, 2008). The methods of crop improvement have changed dramatically through last 25 years. The continued exploitation of biotechnology and the integration of genomic tools in crop improvement widen the plant breeding research. The novel genetic approaches like next generation sequencing (NGS), high-throughput marker genotyping, advanced-backcross QTL analysis, introgression libraries (ILs), multi-parent advanced generation intercross (MAGIC) population, can be harnessed to recognize the genetic variations within the crop species and between cultivated and wild species (Varshney et al., 2009). The seeds of molecular genetics are immensely sprouting and sooner will grow into a self-sufficient fruitful plant to alleviate the world hunger. Application and Impact of Molecular Genetics on Crop Improvement: The Father of Green Revolution, Dr. Norman Borlaug said in an interview: Biotechnology helps farmers produce higher yields on less land. This is a very environmentally favorable benefit. He justified his statement by giving the fact that the worlds grain output in 1950 was 692 million tonnes. After forty years or so later, the worlds farmers sown the crops in about the same acreage of land but they harvested 1.9 billion tonnes that counts the 170% increase in global production. To get this increased production in 1999 using the same conventional breeding, farmers would have needed an additional land of 1.8 billion hectares, instead of using 600 million as used in 1950 (www.actionbioscience.org). Opportunely, plant breeding research is at an arena where there are remarkable advances being made at molecular levels and these endow with opportunities to augment the molecular technologies available for crop improvement as outlined below. Distant hybridization: With the advancement of molecular genetics, it is now possible to transfer genes between distantly related plants. Now genes can be transferred interspecific and intergeneric. Recombinant DNA technology can be amplified to transfer the desirable genes from lower level organisms. For example, Progenies derived from rice (Oryza sativa ssp. japonica) plants pollinated by Oenothera biennis exhibited numerous morphological and developmental traits. Results from amplified fragment length polymorphism (AFLP) analysis showed that several rice lines contained extensive genetic variations, which included disappearance of rice parental bands and/or appearance of novel bands (Chu Xiu Chang et al., 2007). Recombinant DNA technology: There are two methods for crop improvement using r-DNA technology namely, direct and indirect methods. The direct method involves the introduction of novel gene(s) to crops by transformation outside the constraints of sexual crossing. Whereas, the indirect method involves the improving crops by the development of molecular markers (Miflin, CIHEAM). These methods have their specific significance in plant breeding so, are discussed as under. Molecular Markers for crop improvement: Molecular marker refers to the easily detectable marker linked to a desirable trait. The plant breeding analyses like, early generation selection, enrichment of complex F1 generation, choice of donor parent in backcrossing, recovery of recurrent parent genotype in backcrossing, linkage block analysis and selection, exploit molecular markers as a valuable tool. Application of molecular markers in plant breeding includes Germplasm characterization/fingerprinting, determining seed purity, systematic sampling of Germplasm, and phylogenetic analysis. Molecular markers played a key role in replacing bioassays (Varshney et al., 2008). Crop plants exhibit 20-50,000 genes, out of which only few are of our interest in crop improvement. Conventional linkage maps are based on these genes of interest which have distinct morphological effect. But there are limitations in such maps as they cannot map out the genes governing quantitative traits, are time consuming and tedious. Therefore, emphases were made to focus on molecular markers for linkage mapping. There are many types of molecular markers visually isozymes, restriction fragment length polymorphism (RFLP), randomly amplified polymorphic DNA (RAPD), cleaved amplified polymorphic sites (CAPS), simple sequence repeats (SSR), amplified length fragment polymorphism (AFLP), and the latest includes single nucleotide polymorphism (SNP) and single feature polymorphism (SFP), (Bertrand C. Y Collard and David J Mackill, 2008; Varshney et al., 2006). Out of these, SSR or microsatellite is the most widely used marker in major cereal crops (Bertrand C. Y Collard and David J Mackill, 2008). The SNP and DArT (diversity array technology) markers are other high-throughput markers, which can be used to prepare the whole genome map even without the availability of sequence data for the crop (Varshney et al., 2006). In recent years, noteworthy and stimulating progress has been made in marker assisted technology and the development of markers linked to the gene of interest. Some important achievements are given in table 1. Molecular Assisted Selection (MAS): MAS (figure 1) speed ups and makes conventional breeding easier as herein, linked molecular markers are used for indirect selection of desirable traits that were difficult to select earlier in seedling stage (Anushri Varshney et al., 2004). Jonathan et al., 1998, while performing their research on Musa found that due to its triploid nature, the highly relevant generation and the precise linkage maps is not usually attainable. So, to generate linkage map, they first generated traditional linkage map based on diploid relatives and extrapolation to the polyploidy crop and then did polyploidy mapping based on single dose markers in population derived from crosses between tetraploid and diploid genotypes. Thus, it became possible to generate the linkage map of current Musa, which is an allotetraploid. Bioinformatics: Bioinformatics is often overlooked side of molecular marker strategies. As we know that molecular technologies produces a large number of data, with great chances of error introduction during handling and interpretation. The correct accreditation of data is necessary for getting the precise results after a long run of experiments, so for maintaining that huge amount of data bioinformatics enabled such software that provide accurate results and are available through World Wide Web (Simmonds et al., 1999). Quantitative trait Loci (QTL): The most interesting use of molecular markers is the efficient selection of traits. They also made it possible to select polygenic traits controlled by QTLs, by the use of molecular markers like RFLPs and isozymes. Interesting work has been done on QTL by Tanksley et al., on tomatoes, Stuber et al., on maize, McCough and Doerge on rice and Bradshaw et al. on potatoes. The future research can be made in improving the disease resistance in cereal crops (Simmonds et al., 1999). Development of nodules in cereals: Leguminous plants have root nodules that contain nitrogen fixing bacteria known as Rhizobium. These bacteria fix atmospheric nitrogen in root nodules in the form of nitrates. If this gene can be transferred or active root nodules can be developed in cereal crops like wheat, rice, maize, sorghum, pearl millet, barley etc. thus, the reliability of crops on chemical fertilizers would be reduced leading to a significant reduction in cost of production as well as environmental pollution. The introduction of symbiotic biological nitrogen fixation into the major non-legume crops of the world would be one of the most significant contributions that biotechnology could make to agricultural sustainability. With this objective, Edward C. Cocking et al., studied the interaction of rhizobia with the root systems of cereals. They have already confirmed that some naturally occurring rhizobia, such as Parasponia species those isolated from root nodules of non-legume and Aeschynomene from stem nodules of tropical legume species, are able to enter the root systems of maize, rice and wheat by crack entry means the part of plant where lateral roots emerge through the root cortex, so it results to the both inter and intra cellular penetration of rhizobiain, particularly in the cortex of emerging lateral roots. In the recent study, they have interacted oxygen tolerant Azorhizobium caulinodans ORS571 (kindly donated by Dr J K Ladha, IRRI) isolated from stem nodules of the tropical legume Sesbania rostrata with the root systems of rice and wheat. We have found that intracellular invasion of cells of the cortex of roots of both rice (IR42 and Lemont) and wheat (Wembley) results in plants that are active in nitrogen fixation as determined using acetylene reduction assays (Edward C. Cocking et al.). The Plants for future: (Development of transgenic plants) Transgenic plant is the product of genetic engineering, with artificially inserted foreign gene (Adina Breiman and Esra Galun, 1997). The most important tool in plant biotechnology is the transgenic plant production. This technique requires the study of chromosome at molecular levels, genome sequencing and the identification, selection and then insertion of gene of interest form same species in modified form, or related wild species, unrelated species, genus, across kingdom or from microbes (bacteria, virus, fungi). The successful commercialization of first transgenic crop in 1996 is the remarkable achievement in plant breeding and biotechnology. In 2008, globally both the number of countries (25 countries are GM growers) as well as the number of farmers (13.3 million farmers) growing transgenic crops in 125 million hectares of land, has increased significantly (Table-2), (ISAAA web pages). This technology boosts yields, reduce pesticide usage, increase fertilizer efficiency, enhance disease, drought, pest, resistances to various crops. Recently, Sugarbeet RR herbicide resistant variety is grown in USA, gives significant positive results. Another milestone of transgenic breeding is GOLDEN RICE: genetically modified rice that contain bets carotene which can produce vitamin-A, once consumed (Potrykus, 2001). The production of golden rice presents a second green revolution, where certain countries will become the sole producers of major money making crop. It is believed that this would be the most dominating science in future that will play the most crucial role in the crop improvement and can satisfy the growing world hunger. Future prospects: We already have genetically modified crops for various interesting traits, which are developed so far generally involve only the addition of a single gene. Looking to the future, its unclear whether complex traits, which are thought to involve multiple genes, will be amenable to manipulation through genetic engineering (Jonathan Knight, 2003). All these techniques are costly and the research in public sectors is less due to lack of funds. There is the thrust of evolving the more precise, accelerating and cheaper molecular methods that can revolutionized the world agriculture. DNA chip technology and user friendly marker system are the evolving trends in future. For sustainable and self sufficient agriculture, we need carry out continuous efforts in positive direction. There should be the willingness to accept the research and research products globally, politically and socially as well. Then only we can expect second green revolution. Conclusion: We are at the stage of broad and rapid era of science development in all the fields. Time is ripe for the second green revolution which would not only concentrate on increased productivity but also on value added traits to reduce cost of production, pollution dangers and improved quality. Rapid development of plant biotechnology, particularly molecular genetics will serve as the basis for the second green revolution. To speed up the production process economically, to fulfill the aspirations of huge populace, to achieve diversification and adding value to the primary produce so as to make agriculture enterprise farmers as well as environmental friendly. Advanced technologies are expected to materialize many of our expectations in the 21st century. On the other hand, if we arrogantly enjoy the past but ignore the new challenges, or underestimates our capabilities and feel afraid of innovations like GM crops, it is possible that we miss the good opportunities, as said in this Chinese p roverb, Ninety miles is only the half way of a hundred-mile journey. Confucius once said: The passage of time is just like the flow of the river, which goes on day and night, forever. The past glories are the momentum for our new journey; the journey of science, journey of development, journey towards the state of self reliance, while the lessons from the past may teach us to be smarter. We need molecular genetics to make historic contributions to the rejuvenation of the plant breeding and thus the agriculture.

Cosmo Thinks I’M Fat: Images of Feminity in Women’s Magazines

Cosmopolitan magazine is â€Å"the lifestylist for millions of fun fearless females who want to be the best they can be in every area of their lives. † The best-selling magazine in its category, Cosmopolitan has 58 international editions, is published in 34 languages and is distributed in over 100 countries. Despite its popularity, a number of leading researchers have suggested that the material presented in these magazines can be damaging to the mental state of its readers in numerous ways.After examining the latest issue of Cosmopolitan magazine, it appears that while there are several examples of degrading advertisements and features a majority of the ads are neutral or positive in tone and help define a more modern, feminist woman. The November 2010 issue of Cosmopolitan definitely displayed a number of advertisements that displayed overtly sexualized images of women. One ad for Skyy Vodka showed a bottle of the vodka situated suggestively between the legs of a woman weari ng red leather boots.An article about Kary Perry has her laying in bed with ample cleavage with a caption reading â€Å"This cleavage is Cosmo-approved. † Jean Kilbourne contends that advertising content can affect people in deep and possibly hurtful ways. â€Å"Sex in advertisting is pornographic because it dehumanizes and objectifies people, especially women. † (Kilbourne) When people are constantly bombarded with images of objectified people–whether men or women–they are conditioned to see each other in dehumanizing ways.This means that the attitudes that can lead to sexual aggression are normalized. Kilbourne maintains that â€Å"Ads don’t directly cause violence, of course. But the violent images contribute to the state of terror. And objectification and disconnection create a climate in which there is widespread and increasing violence. † Kilbourne shows concerns about two trends in fashion and advertising that she cites as cultural re actions to the women’s movement. The first is the increase in images of violence against women.This is seen in an ad for Belvedere Vodka that depicts a woman blindfolded being led to a martini glass with a toothpick with two olives-a phallic representation. The second reaction Kilbourne notes is the increase of violent images depicting children–especially young girls. An ad for JC Penny showed two very youthful models dressed as young school girls ad looking vulnerable. These ads seem to suggest that the age of the objectification of women does still exist within the advertising world.Although some parts of the magazine may have objectified women featured, there were actually more displaying positive female role models. Advertisements for Dove body mist and deodorant portrayed women of different healthy weights, shapes and ethnicities- not for sexual gratification but to show confidence. Their contender, Secret, used the motto â€Å"When you’re strong, you spar kle. † However, the most positive image might have come from the Nike â€Å"Make Yourself† campaign. There were two ads from this campaign in the latest issue of Cosmo.The first read â€Å"Make yourself- Strong† and showed an image of Olympic Track and Field Medalist Allyson Felix. The second read Make Yourself Proud and featured professional dancer Sofia Boutella. This grouping provides a diversity called for by Wolf If the icon of the anorexic fashion model were one flat image out of a full spectrum in which young girls could find a thousand wild and tantalizing visions of possible futures, the icon would not have the power to hurt them. What this suggests is that even though there were some negative images, the range of role odels helps to allow women to find whatever they need to find themselves within the pages of the magazine. According to Kilbourne, our culture defines femininity and masculinity in a way that narrows accepted gender roles. The popular cult ure usually trivializes [relational skills] in women, mocks men who have real intimacy with women and idealizes a template for relationships between men and women that is a recipe for disaster: a template that views sex as more important than anything else, that ridicules men who are not in control of their women, and that disparages fidelity and commitment.These stereotypes of masculinity are found over and over throughout the magazine. A story about Alex Rodriquez reads â€Å"Right now, there’s no guy sexier than A-Rod. Baseball fans are obsessed with him, and three of Hollywood’s most gorgeous women have fallen for him. † Some other comments throughout the magazine bring to mind disturbing images if they had been aimed at women rather than men. ? Brad Pitt: It’s hard to tell who has more testosterone: Brad or his chisled Megamind character Metroman ? Chris Pine: He’s starring in this month’saction packed flick Unstoppable Oh, did e mentio n he takes his shirt off too? ? Fernando Verdasco: Speaking of shirtlessness, this top-ranked tennis hunk has been posting pec-tastic images of himself on Twitter ? Enrique Iglesias- We have mixed feelings about the fact that he used a fan’s camera during a concert to take a shot of his crotch. Rude! But can we see it? In a context where the subjects were women these comments would be extremely unnerving. The significance of these observations appears to show that while the objectification of women may be on the decline, it is on the incline for men.The fight for equality is succeeding, to the detriment of the male gender. Unfortunately, even Kilbourne minimizes the effect that this can have on men. There is a world of difference between the objectification of men and that of women. The most important difference is that there is no danger for most men, whereas objectified women are always at risk. Sometimes risks are more than just physical. Also, the assumption that all men are larger and/or stronger than women seems just as narrow as many other assumptions made in the past.

Social Issues Topic at a Glance

Social Issues Topic at a Glance Using Social Issues Topic It is simple for a research paper writing service firm to select low standard totally free sources and finish the paper. If you believe you'd be in a position to finish a research paper after going through few sites or studying basic on-line journals, you're making a fool of yourself. On the flip side, if you feel you wouldn't have the ability to think of a great topic, our research paper writing service experts can analyze your interest space and suggest the topic choices that would get the job done for you. If you can't locate a controversial topic on the list that suits your passion, there are a lot of different resources out there. In addition, it impacts my job as a criminal justice professional because I need to stay current in my understanding of the advantages that social media can offer. Just benefit from their expertise, and you're going to relish your college life. Using social media has both health risks and advantages. There is not a simple path to success. Whenever you opt to ask us for expert support, don't hesitate to speak to our support managers. A seasoned professional will make an error-free assignment very quickly and can help you boost your grades. Our customer support will gladly tell you whether there are any special offers at the present time, along with make sure you are getting the very best service our company may deliver. Don't hesitate to ask a librarian for aid. High school isn't free in Kenya. Our study proves that teens who report elevated levels of time spent on social media are somewhat more likely to report internalizing problems per year later. Also, don't forget to include in-text refernces because it's a Harvard style. So changed the entire history of Britain. You can also try to determine which occupations are going to be in demand in a number of decades, for example when you and your fellows graduate from colleges. Already risking their lives in their law enforcement careers, officers discover that it's increasingly hard to continue to keep their private information private in the present social networking climate. You may implement some research on what careers existed in various nations in past. A prudent student doesn't have to cover a sample paper. New York soft drink size limit should be put into place. You are going to have brief feel of the expression paper, which will act as an inducement for you to buy the entire term paper. Poverty This 4-page paper discusses poverty in the USA and the way that it ranks to other countries throughout the world. Writing a research paper even though may appear challenging is a considerable portion of normal student life. Introducing Social Issues Topic At this time you ought to be prepared to pick the topic that you want to write about. If it's more specific, then focus just on that topic and provide a brief summary of the overall situation. Also when picking the topic you want to consider overused ones, as for some common topics won't be so simple to locate some unique info and make it rather intriguing. When you're selecting a topic, you have to be confident that you've considered all the factors. Again, the target of writing a business report will guide the nature of the analysis that needs to be conducted. On the flip side, if you select a controversial topic you have very little interest in, you'll find it quite tough to finish the assignment. If there's absolutely no article on the subject you are attempting to find, attempt to lessen the question and write about a few specific term or situation. There are many articles out there which tell you how you are able to compose a productive report. You ought to be detached and objective in your writing. With these satire topic examples, you may try to create a number of the satire topics to write about all on your own. The first thing he should work on is topic research. Help promote dialogue, rather than debate.

Summary Of The Crucible - 978 Words

Slave, it is a strong word that, according to the Merriam-Webster Dictionary, is defined as, â€Å"Someone who is legally owned by another person and is forced to work for that person without pay.†1 Plutarch describes the leader of the Rebellion (Spartacus the slave) as strong, invincible, and barbaric. Spartacus and his rallied men escaped confinement from the Roman Republic and fought back because they wanted to be free from the horrible situation they were put in. Also, the gladiators were tough and proved how strong of a group they were by defeating many trained Roman soldiers. It is unjust for any human being to be forced to accept that way of life and not be able to have much of a say in the matter. The three arguments that support this are that, Plutarch defends Spartacus and explains that he was barbaric and shows how invincible Spartacus was. The second piece in support of the argument is that the gladiators were under miserable, inhumane circumstances, however, the p opular gladiators could often be wealthy treated like royalty. Spartacus and his men were a feared group that dominated a lot of the Roman soldiers, even though Crassus ended up defeating these trained Romans. Plutarch’s view on how Spartacus and his men fared against Republican Rome had an immense difference from the typical way people thought during the first and second century of Rome. Plutarch had an interesting view opposed to the other patricians of his time. He talks about Spartacus’ character asShow MoreRelatedSummary Of The Crucible 1151 Words   |  5 Pages6. Plot summary â€Å"The Crucible† is a play that takes place in Salem, Massachusetts in 1692. The play starts in the woods, the characters Abigail, Betty, Tituba, Mary Warren, and Mercy Lewis were casting spells in the forest. Samuel Parris catches them in the woods and Betty passes out. 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She thenRead MoreSummary Of The Crucible 1892 Words   |  8 PagesNoemi Boyd Etheridge AP Literature Per. 2 Nov. 25th, 2014 Title: The Crucible Year of Publication: 1953 Author: Arthur Miller Setting and Time Period: Salem Massachusetts during 1692 and 1693 Primary Characters: Abigail Williams - Niece of Reverend Parris, she is leader of the girls in town and has had affair with John Proctor. John Proctor - Husband of Elizabeth and a hard working man; tries to set Abigail straight by exposing the truth between them but is too worried for his reputation. ReverendRead MoreSummary Of The Crucible 2006 Words   |  9 Pagesof a person as an accused or a sinner happens because of the intolerance to any deviation from the society beliefs. This is seen in the play,The Crucible; articles Death Penalty Disgrace and A True Confession. In these articles, the main thing that stands out is that people can be emotionally manipulated because of their conscious. In The Crucible , each and every character was a scapegoat, either to the unproven beliefs or to vengeance. In the case of Death Penalty Disgrace, Mr. Davis was aRead MoreSummary Of Arthur Miller s The Crucible 1443 Words   |  6 PagesStephanie Provenzano 3rd Period 1. Title: The Crucible 2. Author and date of first publication: Arthur Miller, 1953 3. List four main characters with a one-sentence description of each. a. John Proctor, he is a farmer in his mid-thirties living in Salem, as a well respected man in society he sets an example to all of the other citizens; he is married to Elizabeth practice and has 3 sons. b. Abigail, she is a seventeen year old girl who lives with her uncle, Reverend Parris, because her parents